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Week of 7/19
From 2010.igem.org
Contents |
July 19th
- Colony PCR:
-J24679+B1002+K3 -J24679+B1006+K3 -Lux+RBS+B1006+K3 -Lux+RBS+B1002+K3 (The extension time used was a minute and 20 seconds)
- Sent to sequencing:
1)R0010+P0451 3A 2)R0010+P0451 3D
- Gel Order:
-Ladder -1A-E -2A-E -3A-B -Ladder -3C-E -4A-E
- Transform:
-C: Kit Plate 1 Well 3A -T: Kit Plate 1 Well 7A -A: Kit Plate 1 Well 1C
July 20th
- Digests:
-J24679+B1006 (XP) -C0261 (ES) -LUX+RBS+B1006 (XP) -J23108 (ES) -J23116 (ES) -J23100 (ES) -pSB1C3 (EP+Phosphatase)
- Grow up:
-J23116 -J23108
July 21st
- Colony PCR:
1)Lux+RBS+B1006+J23100+C3 2)Lux+RBS+B1006+J23108+C3 3)Lux+RBS+B1006+J23108+C3 redo 4)Lux+RBS+B1006+J23116+C3 5)J24679+B1006A+C0261+C3 6)J24679+B1006C+C0261+C3
- Digest:
-J24679 (ES) -P0451 (XP) -K3 (EP+Phosphatase) -B1006 (XP) -B1002 (XP) -R0010 (ES) -C3+rfp (EP+Phosphatase)
- Ligations:
-J24679+K3 -P0451+R0010 -J24679+B1006 -J24679+B1002 -P0451+R0010 -R0010+C3+rfp
Gel Order:
-Ladder -4B-C -5A-C -6A-C
4C was grown up.
July 22nd
- Colony PCR:
1)J24679+B1006+K3 2)P0451+R0010+RFP+C3 3)J24679+B1002+K3 (The extension time used was a minute and 40 seconds.)
- Gel Order:
-Ladder -Lux+RBS+B1006+J23100+C3 (A-C) -Lux+RBS+B1006+J23108+C3 (A-C) -Lux+RBS+B1006+J23108+C3 redo (A-C) -Lux+RBS+B1006+J23116+C3 (A)
- The colony 3C was grown up.
- Gel Order:
-Ladder -J24679+B1006+K3 (1A-E) -P0451+R0010+RFP+C3 (2A-E)
-Ladder -J24679+B1002+K3 (3A-3E)
- Sent to sequencing:
1)Lux+RBS+B1006+J23116+C3 2)P0451+R0010+RFP+C3(A) (Forward and Reverse) 3)pSB1T3 yellow (Forward) 4) Lux+RBS+B1006 (Reverse)
July 23rd
- Digest:
-J24679 (ES) -B1002 (XP) -B1006 (XP) -Lux+RBS (ES) -C3 (EP+Phosphatase) -K3 (EP+Phosphatase)
- Ligations:
-J24679+B1002 -J24679+B1006 -Lux+RBS+B1002 -Lux+RBS+B1006
