Week of 7/19

From 2010.igem.org

Contents

July 19th

  • Colony PCR:
 -J24679+B1002+K3
 -J24679+B1006+K3
 -Lux+RBS+B1006+K3
 -Lux+RBS+B1002+K3
(The extension time used was a minute and 20 seconds)
  • Sent to sequencing:
 1)R0010+P0451 3A
 2)R0010+P0451 3D
  • Gel Order:
 -Ladder
 -1A-E
 -2A-E
 -3A-B

 -Ladder
 -3C-E
 -4A-E
7-19-10.jpg


  • Transform:
 -C: Kit Plate 1 Well 3A
 -T: Kit Plate 1 Well 7A
 -A: Kit Plate 1 Well 1C


July 20th

  • Digests:
 -J24679+B1006 (XP)
 -C0261 (ES)
 -LUX+RBS+B1006 (XP)
 -J23108 (ES)
 -J23116 (ES)
 -J23100 (ES)
 -pSB1C3 (EP+Phosphatase)
  • Grow up:
 -J23116
 -J23108


July 21st

  • Colony PCR:
 1)Lux+RBS+B1006+J23100+C3
 2)Lux+RBS+B1006+J23108+C3
 3)Lux+RBS+B1006+J23108+C3 redo
 4)Lux+RBS+B1006+J23116+C3
 5)J24679+B1006A+C0261+C3
 6)J24679+B1006C+C0261+C3
  • Digest:
 -J24679 (ES)
 -P0451 (XP)
 -K3 (EP+Phosphatase)
 -B1006 (XP)
 -B1002 (XP)
 -R0010 (ES)
 -C3+rfp (EP+Phosphatase)
  • Ligations:
 -J24679+K3
 -P0451+R0010
 -J24679+B1006
 -J24679+B1002
 -P0451+R0010
 -R0010+C3+rfp

Gel Order:

 -Ladder
 -4B-C
 -5A-C
 -6A-C
7-21-10.jpg

4C was grown up.

July 22nd

  • Colony PCR:
 1)J24679+B1006+K3
 2)P0451+R0010+RFP+C3
 3)J24679+B1002+K3
(The extension time used was a minute and 40 seconds.)
  • Gel Order:
 -Ladder
 -Lux+RBS+B1006+J23100+C3 (A-C)
 -Lux+RBS+B1006+J23108+C3 (A-C)
 -Lux+RBS+B1006+J23108+C3 redo (A-C)
 -Lux+RBS+B1006+J23116+C3 (A)
7-22-10.jpg
  • The colony 3C was grown up.


  • Gel Order:
 -Ladder
 -J24679+B1006+K3 (1A-E)
 -P0451+R0010+RFP+C3 (2A-E)
 -Ladder
 -J24679+B1002+K3 (3A-3E)
7-22-10 001.jpg


  • Sent to sequencing:
 1)Lux+RBS+B1006+J23116+C3
 2)P0451+R0010+RFP+C3(A) (Forward and Reverse)
 3)pSB1T3 yellow (Forward)
 4) Lux+RBS+B1006 (Reverse)


July 23rd

  • Digest:
 -J24679 (ES)
 -B1002 (XP)
 -B1006 (XP)
 -Lux+RBS (ES)
 -C3 (EP+Phosphatase)
 -K3 (EP+Phosphatase)
  • Ligations:
 -J24679+B1002
 -J24679+B1006
 -Lux+RBS+B1002
 -Lux+RBS+B1006
7-22-10 002.jpg



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