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Materials
You will need:
- Deionized water
- Tryptone
- NaCl
- Yeast Extract
Procedure
- Combine 10g tryptone, 5g yeast extract, 10g NaCl, and 950 mL of deionized water into a large beaker/Erlenmeyer flask.
- Mix until dissolved.
- Check to see if the pH is 7.0 using a pH meter. If needed, add 5N NaOH to adjust the pH to 7.0.
- Fill solution to 1L with deionized water.
- Aliquot into smaller glass bottles. (Optional)
- Autoclave.
Purpose
For culturing desired microbes.
References
- Written by David Larsen 9/11/08
- Cold Spring Harbor protocol
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We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!
We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)
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