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Team:Tsinghua/Notebook/8 October 2010
From 2010.igem.org
Module I, Group 1A
Extract plasmid ptv. Transform into competent with pAH69 through electrotransformation. Spread to 0.03% tetracyclin plates.
Module I, Group 2c
Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I.
Double digestion system
eGFP 5ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 11ul
mCherry 4ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 12ul
Kan 4ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 12ul
Incubate at 37 centidegree overnight.
