Team:Queens-Canada/7 July 2010
From 2010.igem.org
July 7, 2010
PCR
Thai and Basia
pSra-10, pOdr-10, pOdr-1, pSrb-6
Program run using TAQ with an extension time of 130 s and annealing temperature of 58 °C.
Gel Electrophoresis
Thai
pSra-10, pCeh-12, pOsm-10, pOdr-10, pOdr-1, pSrb-6
Used 0.4 g agarose in 60 mL TBE. Gel run for 60 mins at 100 V.
Digestion
Yuli and Hao psB1A3, unc-54 3'UTR
Cut using SpeI and XbaI.
Digestion
Chris and Thai and Yuli and Hao
Digested with EcoRI and PstI:
pFlp-1, pHsp-3, eCFP, mCherry, NpHR, ChR2, pStr-220, pStr-1
Digested with XbaI and PstI:
pRab-7, pStr-1, pMec-7
All digests treated with 5 µl of Dpn-1.
Ligation and Heat Shock
Hao and Yuli
pSB1A3, unc-54 3'UTR (Amp)