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Team:Queens-Canada/30 July 2010
From 2010.igem.org
July 30, 2010
Miniprep
Thai
pRab-7::1A3, pMec-7::1A3, unc-54 3'UTR::1A3, pStr-1::1A3
Minprep
Hao and Steve
pSB1A3, pSB1T3, pSB1K3, pSB1C3, pStr-1::1A2, pSip-1::1A3, pRab-7::1A3
Gel Electrophoresis
Thai and Yuli
pSB1A3, unc-54 3'UTR::1A3
Used 0.4 g agarose in 60 mL TBE. Gel run for 45 mins at 100 V.
Gel Electrophoresis
Thai and Yuli
pSB1T3, pSB1K3, pSB1C3, pSip-1::1A3, pRab-7::1A3, pMec-7::1A3, pStr-1::1A2
Gel Electrophoresis
Hao and Yuli
Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 110 V.
Microinjection
Hao
Practiced injections with Sur-5::GFP marker.
