Team:Queens-Canada/30 July 2010

From 2010.igem.org

July 30, 2010

Miniprep

Thai

pRab-7::1A3, pMec-7::1A3, unc-54 3'UTR::1A3, pStr-1::1A3

Minprep

Hao and Steve

pSB1A3, pSB1T3, pSB1K3, pSB1C3, pStr-1::1A2, pSip-1::1A3, pRab-7::1A3

Gel Electrophoresis

Thai and Yuli

pSB1A3, unc-54 3'UTR::1A3

Used 0.4 g agarose in 60 mL TBE. Gel run for 45 mins at 100 V.

Gel Electrophoresis

Thai and Yuli

pSB1T3, pSB1K3, pSB1C3, pSip-1::1A3, pRab-7::1A3, pMec-7::1A3, pStr-1::1A2

Gel Electrophoresis

Hao and Yuli

Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 110 V.

Microinjection

Hao

Practiced injections with Sur-5::GFP marker.