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Team:Queens-Canada/18 August 2010
From 2010.igem.org
August 18, 2010
PCR
Yuli
pGpd-2
Program run using 9:1 FAST:HIFI blend with an extension time of 3 s and annealing temperature of 57 °C.
Digestion
Yuli and Hao
unc-54 3'UTR, pCeh-12, 7::eCFP, pSip-1::eGFP, pGpd-2, ChR2::eYFP, 3
unc-54 3'UTR, pGpd-2, and 1C3 cut with XbaI and PstI. All others cut with EcoRI and PstI.
Heat Shock
Hao
unc-54 3'UTR::1C3, pCeh-12::1C3, NpHR::eCFP::1C3, pGpd-2::1C3, pSip-1::eGFP::1T3, pSB1C3, pSB1T3, eCFP::unc-54 3'UTR::1T3, ChR2::eYFP::unc-54 3'UTR::1T3
Liquid Culture
Yuli and Hao
eYFP, pSra-10, pMec-7, pHsp-3
PCR
Thai
pSrb-6
Program run using 9:1 FAST:HIFI blend with an extension time of 48 s and annealing temperature of 59 °C.
Gel Electrophoresis
Thai
pGpd-2
Used 0.3 g agarose in 45 mL TBE. Gel run for 40 mins at 100 V.
