Team:Queens-Canada/18 August 2010

From 2010.igem.org

August 18, 2010

PCR

Yuli

pGpd-2

Program run using 9:1 FAST:HIFI blend with an extension time of 3 s and annealing temperature of 57 °C.

Digestion

Yuli and Hao


unc-54 3'UTR, pCeh-12, 7::eCFP, pSip-1::eGFP, pGpd-2, ChR2::eYFP, 3


unc-54 3'UTR, pGpd-2, and 1C3 cut with XbaI and PstI. All others cut with EcoRI and PstI.

Heat Shock

Hao

unc-54 3'UTR::1C3, pCeh-12::1C3, NpHR::eCFP::1C3, pGpd-2::1C3, pSip-1::eGFP::1T3, pSB1C3, pSB1T3, eCFP::unc-54 3'UTR::1T3, ChR2::eYFP::unc-54 3'UTR::1T3

Liquid Culture

Yuli and Hao

eYFP, pSra-10, pMec-7, pHsp-3

PCR

Thai

pSrb-6

Program run using 9:1 FAST:HIFI blend with an extension time of 48 s and annealing temperature of 59 °C.

Gel Electrophoresis

Thai

pGpd-2

Used 0.3 g agarose in 45 mL TBE. Gel run for 40 mins at 100 V.