Team:Warsaw/Calendar-Stage1/30 August 2010
From 2010.igem.org
30.08.2010 Monday
Previous day: Set up liquid cultures of E.coli TOP10 with measured constructs [Ania P.]
We used a protocol described here
Testing the strenght of RBS [Ania P.,Cherry]
Preparation of samples by dilluting the bacteria cultures and examining them in flow cytometer (all according the protocol, but each RBS preformed from one independent culture)
Data analysis with Cell Quest and Microsoft Excel[Cherry]
legend:
30-08-2010.001- TOP10 without construct
30-08-2010.007- B0030
30-08-2010.008- B0031
30-08-2010.009- B0032
30-08-2010.010- B0033
30-08-2010.011- B0034
30-08-2010.012- J61100
30-08-2010.013- J61101
30-08-2010.014- J61107
30-08-2010.015- J61117
30-08-2010.016- J61127
cytometer plots:
FSC- Forward Scatter, cell size
SSC- Side Scatter, cell complexity
FL-1- FL-1 sensor, cell fluorescence
Cytometer data processed with Cell Quest:
![](https://static.igem.org/mediawiki/2010/5/50/Rbs.30.08_01.png)
![](https://static.igem.org/mediawiki/2010/1/12/Rbs.30.08_02.png)
![](https://static.igem.org/mediawiki/2010/0/06/Rbs.30.08_03.png)
![](https://static.igem.org/mediawiki/2010/8/84/Rbs.30.08_04.png)
![](https://static.igem.org/mediawiki/2010/0/08/Rbs.30.08_05.png)
![](https://static.igem.org/mediawiki/2010/e/e6/Rbs.30.08_06.png)
Cell Quest data processed with Microsoft Excel:
geo mean- geometric mean of fluorescence of events in FL1
peak channel- nr of channel which recorded the biggest nr of events
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![](https://static.igem.org/mediawiki/2010/c/c9/Rbs.30.08_xls.png)