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Team:Osaka/Notebook
From 2010.igem.org
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| week7 | 5 | 6 | 7 | 8 | 9 | 10 | 11 |
| week8 | 12 | 13 | 14 | 15 | 16 | 17 | 18 |
| week9 | 19 | 20 | 21 | 22 | 23 | 24 | 25 |
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| week11 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| week12 | 10 | 11 | 12 | 13 | 14 | 15 | 16 |
| week13 | 17 | 18 | 19 | 20 | 21 | 22 | 23 |
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| 21 | 22 | 23 | 24 | 25 | 26 | 27 | |
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Notebook
October 1 (Fri)
- Gel electrophoresis (?)
- Ligations
- 006: 004 as upstream, FcenA as downstream, 1-5A as vector
- 007: 005 as upstream, FcenA as downstream, 1-5A as vector
- 010: 004 as upstream, Fcex as downstream, 1-5A as vector
- 011: 005 as upstream, Fcex as downstream, 1-5A as vector
- 036: 032 as upstream, 033 as downstream, 1-5A as vector
- 037: 004 as upstream, 024 as downstream, 1-1C as vector
- 038: 005 as upstream, 024 as downstream, 1-1C as vector
- Transformation of ligation products
- PCR of ENO2 promoter, ADH2 promoter, SUC2 signal sequence
- Parts from NYU team:
| ID | Part Name | Resistance | Description |
|---|---|---|---|
| R1 | <bbpart>BBa_K416003</bbpart> | A,K | yeast secretion tag |
| R2 | <bbpart>BBa_K416004</bbpart> | A | Aga2 yeast cell surface display tag with linker |
- Miniprep of 026, 028, 030
- Restriction digest
- 026, 028 with XbaI, PstI
- 030 with EcoRI, SpeI or EcoRI only
- Gel electrophoresis
- (RESULTS?)
- Transfer to solution culture: 021, 025, 026, 034, 036
TO CHECK/CONFIRM
- 'K1' (mentioned on 9/250 -> where did it come from?
- 9/25 - miniprepped parts (inoculated 9/24) were plasmids from karita sensei? biobrick parts pcr-cloned on 9/23? why where these parts transformed immediately after miniprep?
- 9/24~27 'CelB, Cel44A' etc vs 'Cel5, Cel8, Cel44' which nomenclature refers to what (received plasmids, pcr product?)
