Team:Osaka/week12

From 2010.igem.org


October 10 (Sun)

  1. Electrophoresis
  2. Ligation
    • 048(C) R1 + FcenA
    • 060(C) 001-2 + GeneⅢ
    • 051(C) R2 + 022
    • 066(A) 3-2P + 026
    • 069(A) 049 + 026
    • 070(A) 052 + 026
  3. mini prep
    • 058①,051②,016①,013②
  4. cut check
    • 016①,058①,013②,051②,1-13D,050①,025②
    • PCR Products(ADH2,ENO1)
    • Man→034
    • glr→031
    • ADH2→027
    • ENO→029
    • Cel44A→075
  1. PCR for DNA segment
    • Cel5B 3/4 α-agultinin
  2. To liquid culture
    • 1-13D,1-12M,1-23L,013,056,069-2,061 →culture
  3. Transformation
    • 048,060,051,066,069,070,1-13D

October 11 (Mon)

  1. Transformation
    • 034,031,027,029,075
    • 014-2,021-2,022-2,025-2,047
  2. mini prep
    • 1-23L,1-12M,061①,064-2②,056①②,013①②
    • 1-13D,061②,064-2① →　destruction
  3. cut check
    • 1-12M,064-2②,013①,056①,061①,1-23L
  4. To liquid culture
    • plate 025(10/8),022(10/8) each 2 colony to culture
  5. Electrophoresis
    • 064 ok
    • 061 x
    • 056 x
    • 013 ok
  6. Ligation
    • 048(C) R1 + FcenA
    • 051(C) R2 + FcenA
    • 053(C) R2 + 024
    • 059(C) 001-2 + ToR
    • 068(K) 050 + 026
    • 070(A) 052 + 054
    • 072(K) 049 + 054
    • 076(A) 050 + 054
    • 077(A) 2-4A + R1
  7. cutcheck
    • R2 →XP
    • 1-23L→ E
  8. To liquid culture
    • 1-13D①,070①②,066①②,069①②,060①②,048①②,051①②
  9. Electrophoresis
    • 1-23L ok
    • 036② ok
  10. Ligation
    • 086(C) 024 + 1-23L
    • 078(A) 2-4A + R2
    • 085(C) 023 + 1-23L
    • 084(K) 049 + 1-23L
    • 083(K) 3-2P + 1-23L
    • 082(K) 035 + 1-23L
    • 081(C) 022 + 1-23L
    • 079(C) 036 + 1-23L

October 12 (Tue)

  1. mini prep
    • 1-13D,022-2①②,048①②,060①②,066①②,069①②,070①②
    • 051①→　destrustion
  2. Transformation
    • 047,063,069,0071,078,079,080,081,082,083,084,085
  3. cutcheck
    • 1-13D,022-2,048,066,069,070,1-13D,060
  4. Electrophoresis
    • 1-13D →x
    • 1-13 →x
    • 022-2 →x
    • 048 →OK
    • 060 →?
    • 066 →OK
    • 069 →OK
    • 070 →OK
  5. To liquid culture
    • 034,029,027,019-2,022-2,025-2,021-2,031,075,051,072,070,068,053,059,077,076,048,060,1-23L
  6. cutcheck
    • 1-23L,048
  7. Electrophoresis
  8. .Ligation
    • 030(C) SUC d + 1-3A
    • 087(A) 048 + 026
    • 088(A) 048 + 054
    • 089(C) 2-4A + 054

October 13 (Wed)

  1. mini prep
    • 027,034,029,053,077,060,025①,059,075,021-2,019-2,022-2,068,076,070,072,1-23L
    • 048、025②→destruction
    • 051→x
  2. Treatment of restriction enzyme
    • 027,034,029,053,077,060,025①,059,075,1-23L,021-2,019-2,022-2,068,076,070,072
  3. Transformation
    • 030,087,088,089,
  4. .Electrophesis
    • 014-2 x
    • 021-2 ok
    • 022 x
    • 025 x
    • 027 x
    • 029 x
    • 034 x
    • 053 ok
    • 077 ok
    • 076 ok
    • 075 ok
    • 072  ?
    • 070 ok
    • 068 ok
    • 060  ?
    • 059  ?
    • 059d
  5. .To liquid culture
    • 047①②,063,063,069,071,078,079①②,080,081,082,083,084,085
  6. To liquid culture
    • 019-2②,021-2②,025-2②③,027②,029②,034②
  7. Ligation
    • 095 2-4A + 072
    • 096 053 + 026
    • 097 053 + 054
    • 098 2-4A + 068
    • 099 075 + 026
    • 070 052 + 054
    • 103 1-12M + 1-23L
    • 093 006 + 1-23L
    • 094 007 + 1-23L
    • 091 009 + 1-23L
    • 102 0-12M + 026
    • 051 R2 + FcenA
    • 105 2-4A + 076

October 14 (Thu)

  1. mini prep
    • 019-2,022-2②,025-2②,027,029,034,047①②,069,071,078,080,081,082,083
  2. Transformation
  3. Treatment of restriction enzyme
    • cut check→R2,022-2②,027②,029②,078,*054①,019-2②,069,071,081,075,082,083,019-2②,039,060,080
    • 054①,019-2②,069,071,081,075,082,083,019-2②,039,060,080
    • for Gel extract→047①②,025-2③,034,1-5A,1-1C,1-3A
  4. Electrophoresis
    • R2 ok
    • 019 ok
    • 060 ok
    • 059 ok
    • 071 ok
    • 069 ok
    • 059 ok
    • 078 ok
    • 029 ok
    • 027  ?
    • 022 ok
    • 075 ok
    • 080 x
    • 081 x
    • 082 x
    • 083 x
  5. To liquid culture
  6. Treatment of restriction enzyme
    • 039②,036①,036②,047①,

October 15 (Fri)

  1. mini prep
    • 087①②,088①②,089①②,030①②,079①
    • 051①②,086,085,025-2②,031①～④
  2. Treatment of restriction enzyme
    • 079①,087①,088①,089①,030①,030①
  3. Ligation
    • 081(A,K) 022 + 081
    • 082(A,K) 035 + 1-23L
    • 083(A,K) 3-2P + 1-23L
    • 092(A,K) 011 + 1-23L
    • 101(A,K) 075 + 1-23L
    • 106(C) 2-4A + 064
    • 108(C) 076 + 075
    • 047(K) 039 + 036
  4. Electrophoresis
    • 030 x
    • 089 ok
    • 087 ok
  5. 079 ok
    • 036 ok
    • 039 ok
    • glr ok
  6. To liquid culture
    • 094,099,102,103,105,51②,085②,091,086,025-2④,098,095,051,093
    • 097 x
    • 096 x
  7. Transformation
    • 081,082,083,092,101,047,108,047-2,039,036,106
  8. Ligation
    • 031-2(C) glr + 1-3A
    • 100(C) 039 + 079
    • 100-2(A) 039 + 079

October 16 (Sat)

  1. Transformation
    • 031-2(C),100(C),100-2(A),BYP5013(A)
  2. Ligation
    • 81(C) 2-4A + 087
    • 52 2-4A + 088
    • 098(C) 2-4A + 068
  3. Mini prep
    • 051(new),085,086,091,093,094,095,099,102,103,105
  4. Transformation
    • 120(C),121(C),098(C),2-4A(A)
  5. Treatment of restriction enzyme
    • 2-4A①,051(new),085,086,099,102,103,091,093,094,095,105
  6. Electrophoresis
    • 2-4A ok
    • 103 x
    • 102 ok
    • 099 ok
    • 086 ok
    • 085 x
    • 051 x
  7. culture
    • 101,083,047,092,081,082,106,108,039,036,098,025-2,082,108,039,036
  8. Electrophoresis
    • 091 x
    • 093 ok
    • 094 x
    • 095 x
    • 105 x
  9. mini prep
    • 036①,079①


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Retrieved from "http://2010.igem.org/Team:Osaka/week12"