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Team:Harvard/flavor/flavors
From 2010.igem.org
Flavors
Miraculin
Miraculin is 'flavor inverting' protein, found naturally in the fruit of the plant fruit of Synsepalum dulcificum. Not sweet by itself, miraculin binds to taste receptors on the tongue, possibly altering the structure of the receptors and causing traditionally 'sour' flavors to be received as 'sweet'.
We have obtained, synthesized and BioBricked the DNA sequence of miraculin. This has allowed us to work with it in the usual BioBrick fashion, facilitating insertion into the agrobacterium expression vector.
Preliminary tests in E. Coli have been positive, with our YFP-tagged proteins showing definite expression
Brazzein
Brazzein is a sweet-tasting protein that is found in the Western African fruit, the Oubli. The protein consists of a 191 amino acid sequence. It is sweeter than sugar and is used as an alternative low calorie sweetener.
We have obtained, synthesized and BioBricked the DNA sequence of brazzein. We were then able to flank this sequence with a plant specific promoter and terminator and insert this construct into the agrobacterium expression vector.
Preliminary tests in E. Coli have been positive, with our YFP-tagged proteins showing definite expression and a Western Blot also showing successful expression of Brazzein.
Valencia
We originally tried to extract RNA from Valencia oranges and then make a cDNA library. We then used PCR to amplify the Valencene sequence. Valencene is a gene that codes for a molecule that gives Valencia oranges their citrus flavor and aroma.
Unfortunately, after three attempts, we were not able to obtain a viable cDNA library. We then tried to extract genomic DNA. However, none of our PCR tries worked from the genomic DNA. We therefore began to explore alternate methods for obtaining the valencene gene (i.e. from another lab).
Wintergreen
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wintergreen expression system click to enlarge 
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