Team:Harvard/flavor/parts

From 2010.igem.org


parts and primers

Primers

NameDescriptionSequence
J45004_FWintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primerlink
J45004_RWintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primerlink
J45017_FWintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primerlink
J45017_RWintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primerlink
NOSterm_BB_FPlant specific terminator forward primerlink
NOSterm_BB_RPlant specific terminator reverse primerlink
Stop_NOSterm_BB_FNOSt plant specific terminator with stop codon on endlink
pENTCUP2_BB_FPlant specific promoter forward primerlink
pENTCUP2_BB_RPlant specific promoter reverse primerlink

Parts

NameDescriptionLengthSequence
BBa_K382020Arabidopsis optimized Brazzein195link
BBa_K382021Arabidopsis optimized Miraculin660link
BBa_K382022pENTCUP2 plant specific promoter503link
BBa_K382023NosT plant specific terminator253link
BBa_K382024NosT plant specific terminator with STOP codon256link
BBa_K382025Arabidopsis optimized Miraculin with StrepII N-terminus tag696link
BBa_K382026Arabidopsis optimized Brazzein with StrepII N-terminus tag201link
BBa_K382027Arabidopsis optimized Miraculin with StrepII C-terminus tag696link
BBa_K382028Arabidopsis optimized Brazzein with StrepII C-terminus tag201link

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