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the bacterial uv toggle |
In the beginning, there was a UV Toggle (Collins, 2000). The 2010 MIT iGEM team saw that it was good, and decided to implement the Collins toggle in E.coli to create cells with bistable phenotypes. ![]() Then the team decided to make the signal amplify itself, and record a movie to see its propogation. But the team noticed a circle of cell death where the UV exposure had killed some of the cells in the lawn. They decided to make a pLPTa, a low power toggle that still provided bistability, but required less UV power to induce a toggle switch. By site-directed mutagenesis, the team changed the lambda repressor (cI) gene in the Collins pTSMa to a cI that is more sensitive to cleavage by Rec-A, the enzyme activated by UV light exposure. |