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MIT iGEM 2010

the bacterial uv toggle

In the beginning, there was a UV Toggle (Collins, 2000).
The 2010 MIT iGEM team saw that it was good, and decided to implement the Collins toggle in E.coli to create cells with bistable phenotypes. The team planned for the toggle to control fluorescence and phage polymerization. At first there was a pattern of fluorescence induced by patterned UV exposure -- the first image.

Here we see cells controlled by the Low Power Toggle. The cells fluoresce red with UV induction, but at higher UV levels cell death can be seen in the green field.