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MIT iGEM 2010

the bacterial uv toggle

In the beginning, there was a UV Toggle (Collins, 2000).
The 2010 MIT iGEM team saw that it was good, and decided to implement the Collins toggle in E.coli to create cells with bistable phenotypes. There was a pattern of fluorescence or polymer formation induced by patterned UV exposure -- the first image.

Here we see cells controlled by the Low Power Toggle. The cells fluoresce red with UV induction, but at higher UV levels cell death can be seen in the green field.