Team:Washington/Gram Negative/Design

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T6SS locus

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Design of a Tse2/Tsi2 Toxin/Antitoxin Inducible Circuit

The Tse2/Tsi2 toxin/antitoxin system has a relativly simple circuit design. Tse2 and Tsi2 are present in one operon ( as in Pseudomonas aeruginosa) regulated by the pLux promoter. The LuxR transcriptional factor is constituitively expressed, as no tetR is present to repress the production of LuxR. When 3OC6HSl is present, it binds to LuxR, resulting in the induction of Tse2 and tsi2 production. THe pTet, LuxR, and pTet region of the construct is present in part F2620 (link), easing the contruction of the circuit. Washington Diagram F2620-Tse2-Tsi2.png

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