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Team:Edinburgh/Notebook/Blue light producer
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Revision as of 12:19, 7 October 2010 by Macbereska (Talk | contribs)
Blue Light Producer
13/7/2010
Empty edinbrick vector for control.
- LuxAB + edinbrick ligation.
28/7/2010
an update on LuxAB:
- The transformations of Edinbrick are fine but all the luxABs failed (grew blue, not white on Xgal and did not glow, as of 27/7)/
- Still have some digested LuxAB-ediI so re-did ligation and transformation.
Gel Pic- minipreps
| Lane 1&15 | Lane 2 | Others |
| Ladder | EdiI | LuxAB |
GEL PIC
| Lane 1 | Lane 2 +3 | Lanes 3-6 |
| Ladder | EdiI | LuxAB |
- The ligation had the same gel characteristics as previous luxAb-ediI ligations, e.x. it isn't visivle- not hopeful of this transformation working
29/7/2010
