Team:Newcastle/Meetings/4 June 2010

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Informal Meeting 04.06.2010

Urease tutorial-recap

Accessory genes and nickel incorporation necessary more complicated than we thought.

Software engineering cycle

Requirements -> specification-> design-> implementation -> testing -> maintenance ...link back to the requirements

Specification more detail on the requirements- literature

Urease calcium carbonate, optimise for Bacillis

Increase expression- what we thought!

Biosynthesis of active BS in the Absence of Known Urease Accessory Proteins: paper

Accessory genes required!

Look over papers from tutorial

Sporosarcina pasteurii

Biobrick site symbols

Promoter-ribosome- binding- coding sequence –ribosome binding site-coding-ribosome binding coding- terminator sequences ...and spacers

pSpac

ncbi – find the genome – can see the genes

Implementation using sequencher

Find the sequence of part

Assemble parts

Biobrick friendly restriction sites

Genbank/embl file format

Implementation altered by cloning strategy

Biobricks RFC- request for comments

http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_10:_Draft_standard_for_Biobrick_biological_parts -duplo and lego don’t fit together

AP Rachel: video for lego pipette:http://www.youtube.com/watch?v=FRijFX0BpGI&feature=player_embedded

1st lab week Others modelling Filamentous and spidersilk killswitch

RC

DY

AK

(HS)

2nd lab week Others Modelling glue and lysis quorum sensing

JS

RB

SW

PH

(YE)

Urease both

LAB TRAINING GROUPS:

Group 1 starts 14th June: SW, D.Y AK, (HS); working on Biobrick 2 & 5 (End of crack & signalling system; Glue & lysis)

Group 2 starts 21st June: JS, RB, RC, PH, (YE); working on BioBrick 3 & 4 (Non-target-environment kill switch; Spider silk and filamentous cells)

Team members not in the lab training will be working on modeling.
HS and YE will unfortunately be away for both lab training sessions, hence the brackets