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Contents 1 Wetlab 1.1 General lab work "Materials and Methods" 1.1.1 The Biobrick standard and 3A assemply 1.2 Repressor group 1.3 Terminator group 1.3.1 Work flow 1.4 Protocols 1.4.1 References and resources 2 Anti-Terminator experimental setup 2.1 N-protein plasmid 2.2 References

Wetlab

On this page is described the experiences, procedures and protocols that we have used. Further the experimental process is described for the two parallel tracks, the repressor and terminator part, what have we experienced when working with our parts, and the BB standard.

Protocols For all the methods we have shaped the protocols to the standards and experiences of our lab. We have collected the protocols we used in a comprehensive list below where it is possible to read them in full length. They contain our procedure as well as references. Some time this might be weakly documented when given to us by communication with supervisors.

General lab work "Materials and Methods"

The Biobrick standard and 3A assemply

Repressor group

Terminator group

[ here we should write a short abstract ]

Work flow

Protocols

References and resources

• [ if any]

Anti-Terminator experimental setup

N-protein plasmid

The N protein were isolated from salmonella genomic DNA with specific designed primers. We used the natural occurring RBS site, as a High expression of N have shown non specific anti-termination effect on a global scale on the genome. #References References

References

• NC Franklin, JH Doelling - Am Soc Microbiol "Overexpression of N antitermination proteins of bacteriophages lambda, 21, and P22: loss of N protein specificity." - Journal of bacteriology, 1989