Team:Brown/Notebook/September9

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Thursday, September 9, 2010

Gel

  1. Gary's ladder (13ul)
  2. LovTwo PCR
  3. Mnt (Bglb) PCR
  4. Mnt2 PCR
  5. AraC (Bglb) PCR
  6. AraC2 PCR
  7. CI (Bglb) PCR
  8. CI2 PCR
  9. Our ladder (15ul)

Not enough lovtwo left...

Ran 37min starting 15:03

Rerun of Lovtwo PCR

Lovtap2 (lovtap reporter K191005) + Bgl sites

50.0ul total volume

  • 2ul forward primer
  • 2ul reverse primer
  • 1ul template
  • 20ul dH2O
  • 25ul master mix

Did both K191 and WTlov12 2 as we weren't sure which

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