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Team:Edinburgh/BioBricks
From 2010.igem.org
BioBricks: Genomic BRIDGEs
For this part of the project we will have one main BioBrick, which will be the construct required for the two-step markerless insertion, encompassing cat (chloramphenicol resistance) and sacB (prevents growth on sucrose).
Chloramphenicol resistance has already been well characterised in the registry, so our characterisation has mainly focused on sacB.
In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed E. coli no longer smells!
| Name | Type | Link | Description |
|---|---|---|---|
| cat | Protein Domain | BBa_K322210 | chloramphenicol acetyltransferase (chloramphenicol resistance gene). |
| sacB | Coding | BBa_K322921 | Bacillus subtilis levansucrase, lethal to E. coli in presence of sucrose. |
| tnaA upstream region | Protein Domain | BBa_K322705 | Upstream region of E. coli tryptophanase locus, used for targetting genes to this locus using BRIDGE. |
| tnaA downstream region | Protein Domain | BBa_K322706 | Downstream region of E. coli tryptophanase locus, used for targetting genes to this locus using BRIDGE. |
Composite BioBricks in preparation.
BioBricks: Bacterial BRIDGEs
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| Name | Type | Link | Description |
|---|---|---|---|
| luxAB | Protein Domain | BBa_K322139 | Bacterial luciferase producing blue light. |
| luxCDE | Protein Domain | BBa_K322312 | Codes for a fatty acid reductase complex that makes the fatty acids necessary for LuxAB. |
| lumP | Protein Domain | BBa_K322007 | Lumazine protein to shift the wavelength of LuxAB. |
| Phycobilin synthesis operon | Protein Domain | BBa_K322122 | Harvard 2008's BBa_K098010 in pSB1C3. |
| Phycobilin synthesis operon without terminator | Protein Domain | BBa_K322123 | BBa_K098010 without terminator, allowing addition of cph8. |
| cph8 | Protein Domain | BBa_K322124 | Light sensing protein (chimeric Cph1 light receptor / EnvZ protein) for red light. |
| Firefly luciferase | Protein Domain | BBa_K322124 | Ljubljana 2007's BBa_I712019 in pSB1C3. |
| Firefly luciferase S284T mutant | Protein Domain | BBa_K322246 | Firefly luciferase mutated towards the red spectrum. |
| Firefly luciferase 356K mutant | Protein Domain | BBa_K322246 | Firefly luciferase S284T mutant re-mutated towards the red spectrum. |
| Codon optimised bright firefly luciferase | Coding | BBa_K322451 | Photinus pyralis luciferase mutated for increased bioluminescence. |
Composite BioBricks in preparation.
