"
Team:Edinburgh/BioBricks
From 2010.igem.org
BioBricks: Genomic BRIDGEs
For this part of the project we will have one main biobrick, which will be the construct required for the two-step markerless insertion, encompassing cat (chloramphenicol resistance) and sacB (prevents growth on sucrose).
Chloramphenicol resistance has already been well characterised in the registry, so our characterisation has mainly focused on sacB.
In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed E. coli no longer smells!
| Name | Type | Link | Description |
|---|---|---|---|
| cat | Protein Domain | BBa_K322210 | chloramphenicol acetyltransferase (chloramphenicol resistance gene) |
| sacB | Coding | BBa_K322921 | Bacillus subtilis levansucrase, lethal to E. coli in presence of sucrose |
BioBricks: Bacterial BRIDGEs
***
| Name | Type | Link | Description |
|---|---|---|---|
| Codon optimised bright firefly luciferase | Coding | BBa_K322451 | Photinus pyralis luciferase mutated for increased bioluminescence. |
