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From 2010.igem.org

Contents 1 1:PCR 2 2:Digestion(1) 3 3:Electrophoresis 4 4:DNA extraction 5 5:Digestion(2) 6 6:Electrophoresis 7 8:DNA extraction 8 9:Ligation 9 10:DNA extraction 10 12:Ligation

1:PCR

<Member>
mio,easily

<Sample>

• 2

<Protocol>
See Protocol 2

(temperature in annealing)

• 2:70.0℃

2:Digestion(1)

<Member>
Mariko,mizuki

<Sample>

• 1H:Avr2,FD Buffer
• 2(9/6):Nhe1、M Buffer
• 4(8/30):Avr2,FD Buffer
• 5(8/30):Spe1、M Buffer

<Protocol>
See Prptpcpl 9

3:Electrophoresis

<Member>
Mariko,mio

<Sample>

• PCR products(9/11)
• Digestion products(9/11)

<Protocol>
See Protocol 8

4:DNA extraction

<Member>
mizuki,easily

<Sample>
PCR products(9/11)

<Protocol>
See Protocol 4

5:Digestion(2)

<Member>
easily

<Sample>

• 1(9/11)

<Protocol>
See Prptpcpl 9

6:Electrophoresis

<Member>
Mariko,mizuki

<Sample>
Digestion products

• 1(9/11)
• 4L(8/26)
• 8H

<Protocol>
See Protocol 8

8:DNA extraction

<Member>
mio,mizuki

<Sample>

• 4(9/11)
• 5(9/11)

<Protocol>
See Protocol 4

9:Ligation

<Member>
Mariko,mio

<Sample>

• 4(9/11)
• 5(9/11)

<Protocol>
See Protocol 10

10:DNA extraction

<Member>
Mariko,mio,mizuki

<Sample>

• 1(9/11)
• 2(9/11)

<Protocol>
See Protocol 4

12:Ligation

<Member>
Mariko

<Sample>

• 1(9/11)
• 2(9/11)

<Protocol>
See Protocol 10