Team:TU Delft/16 September 2010 content

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Revision as of 12:03, 24 September 2010 by Ptmvanboheemen (Talk | contribs)

Emulsifier

Yesterdays cultures grown over night. Since Top10 cells activate Lac promoters by themselves, I did a quick and dirty experiment on those cultures.

1 ml culture was spin down for 2 min at max speed.

The supernatant was used in the emulsification assay.

# Description OD600 absorption (493 nm) absorption (493 nm) with Sudan II
1 Top 10 (control) 2.177 2.302
2 Top 10 with 206 1.703 1.862
3 Sup Top 10 (control) 0.013 0.341
4 Sup Top 10 with 206 0.015 0.349

So the conclusion is that there is no difference between the control and the transformed cultures. So we just have to wait until we have the K12 strain.

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