Team:TU Delft/16 September 2010 content

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Emulsifier

Yesterdays cultures grown over night. Since Top10 cells activate Lac promoters by themselves, I did a quick and dirty experiment on those cultures.

1 ml culture was spin down for 2 min at max speed.

The supernatant was used in the emulsification assay.

# Description OD600 absorption (493 nm) absorption (493 nm) with Sudan II
1 Top 10 (control) 2.177 2.302
2 Top 10 with 206 1.703 1.862
3 Sup Top 10 (control) 0.013 0.341
4 Sup Top 10 with 206 0.015 0.349
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