Electrophoresis

Preparation

• ・Sample
• ・Gel （1% gel for fine separation, 2% for rough separation, use 2 % gel for colony PCR)
• ・DNA　loading　dye
• ・DNA　λH200　ladder　mix

Procedure

• 1. Add 1 ul dye for 10 ul sample, and apply to the well.
• 2. Apply 2 ul ladder to the edge well, electrophoresis for 30 minutes.
  • ! DNA chain fewer than 100 bp has danger of over flowing.If you do electrophoresis for 27 minutes, chain of 129 bp will narrowly stay in the gel.
• 3. Dye with EtBr for about 20 minutes.
• 4. Observe by UV.
  • Use “Fujifilm” to cut out. This one needs time to boot-up, so turn on the switch first.