Team:USTC/Notebook/Meetings/12 September 2010
From 2010.igem.org
Jocelyn:
Double Digest pduA in new BBF RFC standard.=> ligate pduA into pduB.
Transformation of biobrick promoter-pdu full length into trans 10 => amplify the signal colony from the LB plate.
Hilde:
Transformation of GFP & RFP
Coating plm. of GFP onto A+ plate
Coating plm. of RFP onto Cl+ plate
Fastdigest cutting of pduABJKNTUV(X,P) & Promotor (S,P) for 3 hours
DNA ligation Kit LONG: Promotor-pduABJKNT (overnight)