Team:USTC/Notebook/Meetings/12 September 2010



Double Digest pduA in new BBF RFC standard.=> ligate pduA into pduB.

Transformation of biobrick promoter-pdu full length into trans 10 => amplify the signal colony from the LB plate.


Transformation of GFP & RFP

Coating plm. of GFP onto A+ plate

Coating plm. of RFP onto Cl+ plate

Fastdigest cutting of pduABJKNTUV(X,P) & Promotor (S,P) for 3 hours

DNA ligation Kit LONG: Promotor-pduABJKNT (overnight)