Team:HokkaidoU Japan/Notebook/August20

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Amplified vector by PCR

Electrophoresis to confirm amplification

Amplified pSB1C3, pSB1T3, pSB1A3 accordingly to the table bellow.

Reagent	Amount
DW	34 uL
10x buffer	5 uL
2mM dNTP	5 uL
25mM MgSO₄	3 uL
Suffix-F	0.5 uL
Prefix-R	0.5 uL
KOD	1 uL
DNA Template	1 uL
Total	50 uL

pSB1T3 failed to amplify

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