Team:Edinburgh/BioBricks

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BioBricks: Genomic BRIDGEs

For this part of the project we will have one main biobrick, which will be the construct required for the two-step markerless insertion, encompassing ''cat'' (chloramphenicol resistance) and ''sacB'' (prevents growth on sucrose).
Chloramphenicol resistance has already been well characterised in the registry, so our characterisation has mainly focused on ''sacB''.

In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed ''E. coli'' no longer smells!

BioBricks: Bacterial BRIDGEs

'''Biobrick #1'''

A brighter luciferase: we had synthesised a version of the Photinus pyralis (Ppy) luciferase with 3 specific amino acid mutations: Ile423Leu Asp436Gly and Leu530Arg. These mutations increase the light intensity of the luciferase by a factor of 12.5.

[[ http://partsregistry.org/Part:BBa_K322451]]

Currently, this part is only present in sequence form. Physical DNA will be submitted shortly.

Reference: Fujii H, Noda K, Asami Y, Kuroda A, Sakata M, Tokida A (2007) Increase in bioluminescence intensity of firefly luciferase using genetic modification. ''Analytical Biochemistry'' '''366''': 131-136