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Team:Newcastle/Meetings/4 June 2010
From 2010.igem.org
| Informal Meeting 04.06.2010 | |
| Urease tutorial-recap | |
| Accessory genes and nickel incorporation necessary more complicated than we thought. | |
| Software engineering cycle | |
| Requirements -> specification-> design-> implementation -> testing -> maintenance ...link back to the requirements | |
| Specification more detail on the requirements- literature | |
| Urease calcium carbonate, optimise for Bacillis | |
| Increase expression- what we thought! | |
| Biosynthesis of active BS in the Absence of Known Urease Accessory Proteins: paper | |
| Accessory genes required! | |
| Look over papers from tutorial | |
| Sporosarcina pasteurii | |
| Biobrick site symbols | |
| Promoter-ribosome- binding- coding sequence –ribosome binding site-coding-ribosome binding coding- terminator | sequences ...and spacers |
| pSpac | |
| ncbi – find the genome – can see the genes | |
| Implementation using sequencher | |
| Find the sequence of part | |
| Assemble parts | |
| Biobrick friendly restriction sites | |
| Genbank/embl file format | |
| Implementation altered by cloning strategy | |
| Biobricks RFC- request for comments | |
| http://Bbf.openware.org/RFC.html -duplo and lego don’t fit together | |
| AP Rachel: video for lego pipette | |
| 1st lab week Others modelling Filamentous and spidersilk killswitch | |
| RC | |
| DY | |
| AK | |
| (HS) | |
| 2nd lab week Others Modelling glue and lysis quorum sensing | |
| JS | |
| RB | |
| SW | |
| PH | |
| (YE) | |
| Urease both
LAB TRAINING GROUPS:
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