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Team:TU Delft/13 September 2010 content
From 2010.igem.org
Emulsifier
Our construct from Mr Gene has arrived! We transformed K12, Top10 and DH5alpha immediately.
Alkane degradation
In order to check if the construct of 028 (J61100-rubA4-J61100-rubR) is correct we did a digestion check.
| # | BioBrick | Cut with | Buffer | BSA | Expected Length (bp) | Correct? |
| 0 | Smartladder (5μl) | |||||
| 2 | 028 | PvuI, AhdI | Buffer 2 (NEB) | BSA (1x) | 370,424,742,854,1165 | |
| 3 | 028 | PvuI | Buffer 2 (NEB) | BSA (1x) | 1596,1959 | |
| 4 | 028 | Uncut | Circulized | |||
| 5 | EZload ladder (5μl |
