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From 2010.igem.org

Emulsifier

Our construct from Mr Gene has arrived! We transformed K12, Top10 and DH5alpha immediately.

Alkane degradation

In order to check if the construct of 028 (J61100-rubA4-J61100-rubR) is correct we did a digestion check. Unfortunately the digestion check was not conclusive.

#	BioBrick	Cut with	Buffer	BSA	Expected Length (bp)	Correct?
0	Smartladder (5μl)
2	028	PvuI, AhdI	Buffer 2 (NEB)	BSA (1x)	370,424,742,854,1165	Not conclusive
3	028	PvuI	Buffer 2 (NEB)	BSA (1x)	1596,1959	Not conclusive
4	028	Uncut			Circulized
5	EZload ladder (5μl)