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Team:Cambridge/Gibson/Protocol
From 2010.igem.org
Gibson Assembly: Introduction
Gibson Assembly is a technique for assembling DNA with short (c. 40 bp) overlapping sequences together. Since these overlapping regions can be easily added by PCR with primers which have added "flaps", any DNA sequences can be joined by this mechanism.
Advantages
- No scar created - useful fusion proteins and adding an RBS, where scars can be problematic.
- Can religate linear DNA to a circle - useful for site-directed mutagenesis
- Faster than Biobrick assembly, and works with less DNA.
