Team:Washington/Protocols/EnzymeAssayCapD
From 2010.igem.org
Enzyme Assay for CapD + CapDcp
Run one transpeptidase reaction and one hydrolysis reaction per enzyme Prepare Transpeptidase Reactions
Prepare Hydrolysis Reactions
Final Concentrations
- Enzyme (CapD) 5nM (0.00025mg/mL)
- Amino Acid (L-Glutamate)0 or 5mM
- Substrate 50nM
- HEPES (7.4pH) 25mM
- 0.1% Tween
- Final Reaction Volume 100uL