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Team:Washington/Project/Safety/Secretion
From 2010.igem.org
Our work on Gram-negative Therapeutics was done only on non-pathogenic strains. We used the Type VI Secretion System from Pseudomonas aeruginosa PAO1. All modifications and testing of plasmids and fosmids were in non-pathogenic E. Coli strains, namely EPI300, SW102, and BL21. And Team Tse2's strain...MPI??
The toxin we work with, Tse2, targets only prokaryotic cells, thus posing no danger.
