Team:UNIPV-Pavia/Calendar/September/settimana2

September, 6th

Inoculum of I47, I48, I49 into 5 ml LB+Amp for TECAN experiment of the following day.

• Inoculum of I44-2 and I51-3 into 5 ml LB+Cm34 to evaluate MiniPrep yields.
• Inoculum of I44-2 and I51-3 into 5 ml LB+Cm12,5 to evaluate MiniPrep yields and for sequencing.
• Inoculum for MiniPrep and sequencing of:
  • <partinfo>BBa_J23100</partinfo>_4C5
  • <partinfo>BBa_J23101</partinfo>_4C5
  • <partinfo>BBa_J23105</partinfo>_4C5
  • <partinfo>BBa_J23106</partinfo>_4C5
  • <partinfo>BBa_J23110</partinfo>_4C5
  • <partinfo>BBa_J23114</partinfo>_4C5
  • <partinfo>BBa_J23116</partinfo>_4C5
  • <partinfo>BBa_J23118</partinfo>_4C5
  • I7-A
  • I8-5-D

Inoculum of cultures for TECAN test on self-inducible promoters:

• I14-1
• I15-1
• I16-1
• I17-1
• I18-1
• I19-1
• I14_4C5-2
• I15_4C5-2
• I16_4C5-1
• I17_4C5-1
• I18_4C5-1
• I19_4C5-1
• I6-3
• I3-1
• <partinfo>BBa_B0031</partinfo>

Glycerol stocks of MG43, MC42, MC43. Static TECAN test (absorbance, green fluorescence and red fluorescence) on 200 ul of these cultures.

Cultures were diluted in 4 ml final of LB in order to obtain the same lowest OD with the following formula: culture to remove and substitute with LB=FinalVolume-(lowestOD/misuredOD)*FinalVolume. Centrifuge at 4000 rpm, 25°C for 5 min to pellet the culture, discard the surnatant and resuspend the pellet with 1 mL of M9. Static TECAN test (absorbance, green fluorescence and red fluorescence) on 200 ul of these cultures.