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Team:Washington/Project/Safety/Secretion
From 2010.igem.org
Our work on Gram-negative Therapeutics was done only on non-pathogenic strains. We used the Type VI Secretion System from Pseudomonas aeruginosa PAO1. All modifications and testing of plasmids and fosmids were in non-pathogenic E. Coli strains, namely EPI300, SW102, and BL21. And Team Tse2's strain...MPI??
The toxin we work with, Tse2, is targeted only to prokaryotic cells so does not pose any danger.
