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Contents 1 rocF 1.1 Aims 1.2 Materials and protocol 1.3 Results 1.3.1 Nanodrop 2 Subtilin Immunity BioBrick 2.1 Aims 2.2 Methods 3 Sucrose/Levan's glue 3.1 PHOTOS

rocF

Aims

rocF miniprep: we aim to purify rocF in p.....

Materials and protocol

See Mini prep protocol

Results

Nanodrop

rocF

Concentration of DNA in ng/µl

1. 93.1
2. 102.0
3. 99.4
4. 101.0
5. 98.8
6. 104.7
7. 93.1
8. 57.9
9. 81.9

Subtilin Immunity BioBrick

Aims

Overnight cultures of all the colonies from the 'SI 1/9/10' plates was carried out today.

Methods

The Growing Overnight Cultures protocol was followed. The colonies were grown on LB with chloramphenicol. Altogether 16 colonies were produced on the plates and were left overnight.

Sucrose/Levan's glue

The sucrose cultures and controls were compared. The cultures grown on sucrose should be considerably thicker. The overnight cultures of the different strains were also grown on solid agar plates with and without sucrose.

PHOTOS