Mutate DNA

OUTLINE

Order Olglionucleotides with the desired mutation

Create ssDNA of wild type gene

Allow Olglios to annele to the ssDNA

Use polymerase and DNTPs to synthesize the rest of the gene

Grow Protein

Transform E. coli with the mutant plasmid

Grow and OD until desired OD is reached

Induce bacteria to produce our protein

Grow for 24 hours

Harvest Protein