Team:UNIPV-Pavia/Calendar/September/settimana2

From 2010.igem.org

Revision as of 08:27, 7 September 2010 by Susanna (Talk | contribs)


SEPTEMBER: WEEK 2



September, 6th

Inoculum of I47, I48, I49 into 5 ml LB+Amp for TECAN experiment of the following day.


  • Inoculum of I44-2 and I51-3 into 5 ml LB+Cm34 to evaluate MiniPrep yields.
  • Inoculum of I44-2 and I51-3 into 5 ml LB+Cm12,5 to evaluate MiniPrep yields and for sequencing.
  • Inoculum for MiniPrep and sequencing of:
    • <partinfo>BBa_J23100</partinfo>_4C5
    • <partinfo>BBa_J23101</partinfo>_4C5
    • <partinfo>BBa_J23105</partinfo>_4C5
    • <partinfo>BBa_J23106</partinfo>_4C5
    • <partinfo>BBa_J23110</partinfo>_4C5
    • <partinfo>BBa_J23114</partinfo>_4C5
    • <partinfo>BBa_J23116</partinfo>_4C5
    • <partinfo>BBa_J23118</partinfo>_4C5
    • I7-A
    • I8-5-D

Inoculum of cultures for TECAN test on self-inducible promoters:

  • I14-1
  • I15-1
  • I16-1
  • I17-1
  • I18-1
  • I19-1
  • I14_4C5-2
  • I15_4C5-2
  • I16_4C5-1
  • I17_4C5-1
  • I18_4C5-1
  • I19_4C5-1
  • I6-3
  • I3-1
  • <partinfo>BBa_B0031</partinfo>


September, 7th

MiniPrep for:

CultureQuantifications (ng/ul)
<partinfo>BBa_J23100</partinfo>_4C5
<partinfo>BBa_J23101</partinfo>_4C5
<partinfo>BBa_J23105</partinfo>_4C5
<partinfo>BBa_J23106</partinfo>_4C5
<partinfo>BBa_J23110</partinfo>_4C5
<partinfo>BBa_J23114</partinfo>_4C5
<partinfo>BBa_J23116</partinfo>_4C5
<partinfo>BBa_J23118</partinfo>_4C5
I7-A
I8-5-D
I44-2 (Cm 34)
I51-3 (Cm 34)
I44-2 (Cm 12,5)
I51-3 (Cm 12,5)

Cultures for TECAN test were diluted in the morning (h10:20) 1:100 (40ul in 4ml) and let grown at 37°C 220 rpm till they reach the desired OD.

September, 8th

September, 9th

September, 10th

September, 11th