Team:LMU-Munich/Notebook/Protocols/17 Special thermal cycler programs/Touch62

PCR program: Touch-Down

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95°C	1 min
95°C	30 sec
62°C	30 sec
72°C	30 sec
return to step 2 for 2 cycles
95°C	30 sec
60°C	30 sec
72°C	30 sec
return to step 6 for 2 cycles
95°C	30 sec
58°C	30 sec
72°C	30 sec
return to step 10 for 2 cycles
95°C	30 sec
56°C	30 sec
72°C	30 sec
return to step 14 for 2 cycles
95°C	30 sec
54°C	30 sec
72°C	30 sec
return to step 18 for 2 cycles
95°C	30 sec
52°C	30 sec
72°C	30 sec
return to step 22 for 2 cycles
95°C	30 sec
50°C	30 sec
72°C	30 sec
return to step 26 for 2 cycles
95°C	30 sec
55°C	30 sec
72°C	30 sec
72°C	10 min
12°C	forever