Team:LMU-Munich/Notebook/Protocols/17 Special thermal cycler programs/Touch62

From 2010.igem.org


PCR program: Touch-Down

95°C 1 min
95°C 30 sec
62°C 30 sec
72°C 30 sec
return to step 2 for 2 cycles
95°C 30 sec
60°C 30 sec
72°C 30 sec
return to step 6 for 2 cycles
95°C 30 sec
58°C 30 sec
72°C 30 sec
return to step 10 for 2 cycles
95°C 30 sec
56°C 30 sec
72°C 30 sec
return to step 14 for 2 cycles
95°C 30 sec
54°C 30 sec
72°C 30 sec
return to step 18 for 2 cycles
95°C 30 sec
52°C 30 sec
72°C 30 sec
return to step 22 for 2 cycles
95°C 30 sec
50°C 30 sec
72°C 30 sec
return to step 26 for 2 cycles
95°C 30 sec
55°C 30 sec
72°C 30 sec
*************** ***************
72°C 10 min
12°C forever