Team:LMU-Munich/Notebook/Protocols/17 Special thermal cycler programs/Touch down

From 2010.igem.org

PCR program: Touch-Down

95°C	1 min
95°C	30 sec
56°C	60 sec
72°C	30 sec
return to step 2 for 2 cycles
95°C	30 sec
54°C	60 sec
72°C	30 sec
return to step 6 for 2 cycles
95°C	30 sec
52°C	60 sec
72°C	30 sec
return to step 10 for 2 cycles
95°C	30 sec
50°C	60 sec
72°C	30 sec
return to step 14 for 2 cycles
95°C	30 sec
48°C	60 sec
72°C	30 sec
return to step 18 for 2 cycles
95°C	30 sec
46°C	60 sec
72°C	30 sec
return to step 22 for 2 cycles
95°C	30 sec
44°C	60 sec
72°C	30 sec
return to step 26 for 2 cycles
95°C	30 sec
42°C	60 sec
72°C	30 sec
return to step 30 for 2 cycles
95°C	30 sec
48°C	60 sec
72°C	30 sec
return to step 34 for 30 cycles
72°C	10 min
12°C	forever