From 2010.igem.org
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PCR program: Touch-Down
| 95°C
| 1 min
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| 95°C
| 30 sec
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| 56°C
| 60 sec
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| 72°C
| 30 sec
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| return to step 2 for 2 cycles
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| 95°C
| 30 sec
|
| 54°C
| 60 sec
|
| 72°C
| 30 sec
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| return to step 6 for 2 cycles
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| 95°C
| 30 sec
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| 52°C
| 60 sec
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| 72°C
| 30 sec
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| return to step 10 for 2 cycles
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| 95°C
| 30 sec
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| 50°C
| 60 sec
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| 72°C
| 30 sec
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| return to step 14 for 2 cycles
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| 95°C
| 30 sec
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| 48°C
| 60 sec
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| 72°C
| 30 sec
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| return to step 18 for 2 cycles
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| 95°C
| 30 sec
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| 46°C
| 60 sec
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| 72°C
| 30 sec
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| return to step 22 for 2 cycles
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| 95°C
| 30 sec
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| 44°C
| 60 sec
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| 72°C
| 30 sec
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| return to step 26 for 2 cycles
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| 95°C
| 30 sec
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| 42°C
| 60 sec
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| 72°C
| 30 sec
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| return to step 30 for 2 cycles
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| 95°C
| 30 sec
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| 48°C
| 60 sec
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| 72°C
| 30 sec
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| return to step 34 for 30 cycles
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| 72°C
| 10 min
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| 12°C
| forever
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