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From 2010.igem.org

Modelling

Taq DNA Polymerase

Taq Polymerase with no mutations

Aliphatic Index: 98.33

Ext Coefficient: 112760

Theoretical PI: 6.04

Instability Index: 32.93

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We redesigned TAQ so it is suitable to undergo ligation independent cloning, to do this we changed the N and C terminal.

This is the redesigned Taq Polymerase

Aliphatic Index: 96.46

Ext Coefficient: 107260

Theoretical PI: 6.09

Instability Index: 33.39

The wild type Taq Polymerase was changed at the N and C terminal, with the Root Mean Square Deviation of 6.8834. This number indicates the measure of the average distance between the backbones of the two superimposed proteins.

The picture below shows the differences between the wild type Taq Polymerase and the redesigned Taq.

(Green is the redesigned Taq and Pink is the wild type Taq)

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Our Taq Polymerase with mutations

485 Asparagine - Valine

515 Serine - Leucine

540 Lysine - Leucine

659 Arginine - Isolucine

663 Lysine - Leucine

Aliphatic Index: 98.2

Ext Coefficient: 107260

Theoretical PI: 5.91

Instability Index: 32.91

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