Uh-oh...is this an omen for things to come?

Raida

Today, I set up a PCR Reaction in order to biobrick MalE and MalE31 without the signalling sequence. I set up 8x with the MalE-BBKdelss-F and the MalE-R primers. The negative control is a MalE plasmid. Another set of reaction was set, which is with the MalE-BBk-F and the MalE-R and the positive control was MalE plasmid.

I have also completed my draft with suggestion made from Himika and Paul on my introduction and also which section should have the paragraphs that I had written. I also wrote for the Legal portion of the paper.

Emily

Today I ligated my digests from yesterday and then transformed them into TOP10 competant cells. I plated on AK plates and left for overnight growth. I also finally induced some fo my ovenright cultures with Arabinose, so we shall get the results form the plate reader tomorrow. Today I also verified sequeuncing results from the DegP constructions (both the DegP consructs are now built and sequenced!). Today I also worked on editing some of the minireports for the Wiki.

Jeremy

Today is my first real day of wetlab stuff since my time off. I redid the pRFP biobrick and will run the gel tomorrow. I also did a transformation of MalE, MalE31 and NlpE into the Ravio competent cells. I also made more agar and broth.

Non-wetlab stuff that I did included fixing areas of the wiki, writing up the transcription/translation portion of the circuit and doing the Calgary, University of Calgary and O'brien sections of the wiki.

Patrick

Trying very hard to get a strand of amino acids (i.e. a very long cylinder object) to twist and fold into a "protein" (i.e. a very long cylinder object knotted together). Skeleton and inverse kinematic animation is proving much more difficult than it first appears to be.

Chris

Today, I did plasmid preparations of the construction of (I0500-I13507 which will be given to Dev to verify. As well, I made ampicillin containing plates and made overnight cultures of the CpxP promoter and the ibpAB-fsxA-T7 fusion promoter inserted into the AK and AC plasmid. I continued my reading into DegP and Cpx which the paper was due last week...I will get that to you as soon as possible, Emily.