Chris

Today, I ligated the restriction digests that were set up yesterday including the insertion of CpxP and ibpAB into ampicillin-kanamycin and ampicillin-chloramphenicol plasmids. They were plated on just chloramphenicol and kanamycin plates so as not to induce as much stress on the cells. As well, I helped Himika book the hotel at which we are staying at for iGEM and got quotes on our flights to Boston in November. I also wrote up a new draft of the budget for our team this year with actual numbers, showing we are actually in green!!

Emily

Today I set up a digested of my minipreppoed hypothetical Biobricked malE with EcoRI/PstI. I was looking to get bands at ~3 KB for the psB1AK3 vector and ~1.2 for the malE insert. Unfortunately I only got one band, which either indicates that the digestion did not work and malE could be in the vector, or that there is nothing in the vector and the single band is the cut AK vector. I ran another digest, using the same enzymes with J13002-LuxOD471-B0015 from last year, and using different enzymes with malE.